| 1. | The chimera embedding technique is presented for the external store separation configuration . it divides the whole region into many subdomains . euler equations are used for every subregion with the finite volume method
本文采用嵌套网格技术，将飞机投放外挂物的模型分成多个计算子域，对飞机投放外挂物的复杂外形进行了分区迭代求解。 |
| 2. | The research of the renaturation and biological activity of the chimeras confirmed the active sites of hwtx - i and mviia further . it also indicates that the structure of ick is stable and is an ideal model molecule for protein engineering
通过对嵌合体复性和活性情况的研究，进一步证实了hwrtx一i和mvlla的活性位点;并且表明抑制剂胧氨酸结模体( ick )的结构确实是相当稳定的，是一种进行蛋白质工程的较理想的模型 |
| 3. | In this paper based on the optimization design method using control theory for airfoils , a design method for multi - element airfoils is developed with the chimera grids . and optimization computation for reducing drag of shockwave of single airfoil in transonic flow is also successful
本文的主要工作是：以在控制理论进行单段翼型优化设计的基础上，利用嵌套网格技术将其发展为适用于带襟翼多段翼型的优化设计。 |
| 4. | Methods : the two pairs of designed primers were based on pzp3 a and hcg p - ctp109 - 145 cdna sequences . the pzp3 a - hcg p - ctp109 - 145 chimera was amplifiled by overlapping pcr . the chimera was cloned into ppic9k plasmid and transformed into e . coli dh5 a
结果： 3步pcr扩增出pzp3 - hcg - ctpdna片段，插入到载体质粒ppic9k的克隆位点，获得重组ppic9k - pzp3 - hcg - ctp表达质粒，测序结果显示插入序列与设计预期完全一致。 |
| 5. | Strain bl21 , and gene expression was induced by iptg . the target proteins were directed into the periplasmic space by the staphylococcal protein a signal sequence preceding the rgd - hirudin gene . using ion exchange chromatography and gel filtration chromatography , the chimera proteins were purified , and both of them showed a single band in tricine - sds - page . the results of activity analysis suggested that these two chimera proteins not only have antithrombin activities , but gain platelet aggregation inhibitory activities as well
通过离子交换层析和凝胶过滤层分别对两种嵌合体蛋白进行纯化，纯化产物在tricine - sds - page中都显示为单一条带。活性分析结果表明两种嵌合体蛋白在保留水蛭素抗凝血酶活力的同时，还呈现抗血小板聚集活性。 |
